Production and characterization of monoclonal antibodies for the detection of the hepatitis C core antigen

Background: Despite highly effective treatments to cure hepatitis C, almost 80% of chronically HCV-infected people are not treated, as they unaware their infection. Diagnostic rates and linkage care must be substantially improved reverse this situation. The HCV core antigen (HCVcAg) is a conserved protein that can detected in the blood patients indicates active Aim: To produce murine monoclonal antibodies against HCVcAg suitable for rapid inexpensive tests detect Methods: BALB/c mice were sequentially inoculated with purified recombinant from Gt1a, Gt3a, Gt4a, Gt1b genotypes. Hybridomas producing desired selected, reactivity various genotypes was tested by Western blotting dot blotting. binding kinetics analyzed surface plasmon resonance (SPR), ability double antibody sandwich ELISA (DAS-ELISA). Results: Four specific (1C, 2C, 4C, 8C) obtained. 1C, 4C recognized all (Gt1a, Gt1b, Gt2a, Gt4a), while 8C did recognize Gt2a Gt3a Based on SPR data, antibody-HCVcAg complexes formed stable, 2C having strongest properties. DAS-ELISA different combinations easily culture supernatants cells. Conclusion: Specific cross-reactive anti-HCVcAg strong properties obtained may useful detecting samples.